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Evidence Guide: MSL973029 - Perform histological procedures

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL973029 - Perform histological procedures

What evidence can you provide to prove your understanding of each of the following citeria?

Cut sections of tissue

  1. Label required number of microscope slides in accordance with workplace traceability requirements
  2. Check the flotation bath is ready and satisfactory for use
  3. Secure block in microtome following specified safety directions
  4. Cut representative sections at the required thickness observing prescribed safety measures
  5. Float sections onto water bath to flatten tissues and pick up sections onto microscope slides ensuring identification on slides matches that on the block
  6. Compare the blocks and sections ensuring the tissue on slides matches that in the blocks
  7. Apply procedures to prevent cross-contamination between samples
  8. Inspect sections and reject any that do not meet quality control standards
  9. Label specimens in accordance with workplace traceability requirements
  10. Select reagents specified in workplace procedures
  11. Stain sections according to workplace procedures
  12. Mount sections ensuring no bubbles
  13. Examine sections microscopically to ensure expected staining outcomes have been achieved
  14. Inspect slides and reject any that do not meet quality control standards
Label required number of microscope slides in accordance with workplace traceability requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check the flotation bath is ready and satisfactory for use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Secure block in microtome following specified safety directions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut representative sections at the required thickness observing prescribed safety measures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Float sections onto water bath to flatten tissues and pick up sections onto microscope slides ensuring identification on slides matches that on the block

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Compare the blocks and sections ensuring the tissue on slides matches that in the blocks

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between samples

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect sections and reject any that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Label specimens in accordance with workplace traceability requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select reagents specified in workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Stain sections according to workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Mount sections ensuring no bubbles

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Examine sections microscopically to ensure expected staining outcomes have been achieved

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect slides and reject any that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a safe work environment

  1. Ensure personal safety and minimise cross-contamination using personal protective equipment (PPE)
  2. Handle chemicals and sharps safely
Ensure personal safety and minimise cross-contamination using personal protective equipment (PPE)

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Handle chemicals and sharps safely

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge